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Biotech / Medical : biotech firesales -- Ignore unavailable to you. Want to Upgrade?

To: tuck who wrote (1087)	7/28/2006 12:58:30 PM
From: scaram(o)uche	Read Replies (1) \| Respond to of 3661

Pizzas and coffee? Huh? O.K. Don't know why, but..... good. :-) I'm pulling for gtop. Part of the problem in giving it a true test was that Idec ended up switching to a one-bottle strategy for all patients, switched to a panel of anti-id's that were most common among lymphomas. The patient-specific aspect has never been given an effort like this one, and the use of a carrier conjugate and GM-CSF conjugate means that everything that Levy could have thrown at the idea WAS thrown at the idea. the answer in Vacaville, when we asked about antigen escape? vaccinate 'em again! :-) J Immunol Methods. 2003 Jul;278(1-2):95-104. Individualized human scFv vaccines produced in plants: humoral anti-idiotype responses in vaccinated mice confirm relevance to the tumor Ig.McCormick AA, Reinl SJ, Cameron TI, Vojdani F, Fronefield M, Levy R, Tuse D. Large Scale Biology Corporation, Vacaville, CA 95688, USA. alison.mccormick@lsbc.com We have developed a method for rapidly producing in plants the idiotype regions of the tumor-specific Ig as single-chain Fv (scFv) proteins for use in the treatment of non-Hodgkin's lymphoma. Variable region gene sequences were generated from either a tumor hybridoma or human tumor biopsy cells, and idiotype domains were joined by a novel linker and cloned into a modified tobacco mosaic virus (TMV) vector designed to secrete the scFv protein in infected Nicotiana benthamiana plants. Thirty-eight out of 44 human scFv proteins showed Coomassie visible material in crude secretory (interstitial fluid, IF) extracts, 21 of those between 100 and 800 microg/ml. Eight of these proteins were tested for appropriate idiotype responses in vaccinated mice. In all eight cases, anti-idiotype immune responses were induced with minimal cross reactivity to irrelevant Ig or scFv proteins. Four out of four anti-scFv sera were also shown to recognize the Ig on human tumor cells by flow cytometry analysis.