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Biotech / Medical : Ebola Outbreak 2014 - News, Updates and Related Investments -- Ignore unavailable to you. Want to Upgrade?

To: Rocket Red who wrote (124)	8/10/2014 2:44:46 PM
From: Rocket Red	Read Replies (1) \| Respond to of 608

pDC316 mbx vaccine Adenoviral construction Adenoviral vectors containing p47(S303304328D), lacZ, p67(V204A), and PAK1(K298A) were constructed as previously described, 13 , 14 and expression was confirmed in HUVECs by immunoblot (not shown). Ad-VE-cadherin GFP was created by subcloning the VE-cadherin GFP into the shuttle vector pCD316(io) (Microbix; Toronto, ON, Canada). After cotransfection with pBHGlox(del)E1,3Cre in 293IQ cells (Microbix), recombinant viruses were harvested, cloned, and titered. Expression was confirmed with microscopy and immunoblot (not shown). bloodjournal.org