To: the Chief who wrote (2379 ) 4/15/2021 8:23:31 AM From: tturner Read Replies (1) | Respond to Exposure to GO concentrations >50 µg/ml resulted in A549 cytotoxicity, while concentrations >20 µg/ml were found to be cytotoxic to human fibroblasts and lung epithelial cells (30 ). The results of the present study revealed that the injection of lower doses of GO (5 and 10 mg/kg) via the tail vein did not induce significant lung edema, vascular permeability or histopathological changes. In addition, low doses exerted no significant effect on oxidative injury and inflammatory reactions in the lung. However, higher concentrations of GO (50 and 100 mg/kg) induced lung edema and increased lung vascular permeability and histopathological changes, as well as triggered oxidative injury and inflammation These results indicate that the toxic effects of GO are dose-dependent. This phenomenon has also been reported in previous studies. In an in vivo experiment on mice, low- (0.1 mg) and medium- (0.25 mg) level exposure to GO was not associated with significant toxicity, but high-level exposure (0.4 mg) was chronically toxic ( 31 ). Furthermore, inflammatory cell infiltration, granulomatosis and pulmonary edema were observed in the lungs of mice injected with 10 mg/kg GO, whereas those injected with 1 mg/kg excreted the GO after 1 week, and no significant pathological response was observed ( 32 ). Zen stuff is used topicaly.. not injected and this study used milli grams, Zen uses micro grams.... believe there are 2 micro grams per mask. I doubt if all 2 micro grams get to the lungs. Granted, HC would want a bench mark on how much could cause problems, but zen should be out of the woods when all is said and done.
