The Endostatin Patent (pending - for those interested;
others skip it).
A collagen XVIII fragment as an inhibitor of angiogenesis and its therapeutic uses in the treatment of angiogenesis-dependent cancers. O'Reilly, Michael S.; Folkman, M. Judah. (Children's Medical Center Corporation, USA). PCT Int. Appl., 83 pp. CODEN: PIXXD2. WO 9715666 A1 970501.
Designated States: W: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, UZ, VN, AM, AZ, BY, KG, KZ, MD, RU, TJ, TM. Designated States: AT, BE, BF, BJ, CF, CG, CH, CI, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE. Patent written in English. Application: WO 96-US16925 961023. Priority: US 95-5835 951023; US 96-23070 960802; US 96-26263 960917; US 96-740168 961022 CAN 126:338845
Abstract
Endostatin, an inhibitor of endothelial cell proliferation that is capable of inhibiting angiogenesis and causing tumor regression is described for therapeutic use. The protein has potential therapeutic use in a no. disease assocd. with abnormal angiogenesis. Endostatin is approx. 20 kDa and corresponds to a C-terminal fragment of collagen type XVIII, and methods of treating angiogenesis-related disease. The protein was identified in conditioned medium from the hemangioendothelioma cell line EOMA using inhibition of proliferation of cultured endothelial cells. Inhibition was specific to endothelial cells. The protein was manufd. either in Escherichia coli or using a baculovirus expression system using a coding sequence derived from the mouse type XVIII collagen gene. The growth of Lewis lung carcinoma metastases was almost completely suppressed by systemic administration of endostatin at 0.3 mg/kg/day.
Patent Classifications; Main IPC: C12N015-12. Secondary IPC: C07K014-78; C07K016-18; A61K038-39; A61K038-48.
And the next paper worth reading:
Tumor regression after endostatin therapy. Black, William R.; Agner, R. Christopher. Dep. Internal Med. and Med. Oncology, Rowan Regional Med. Cent., Salisbury, NC, 28144, USA. Nature (London) (1998), 391(6666), 450. |
