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Microcap & Penny Stocks : Computerized Thermal Imaging CIO (formerly COII) -- Ignore unavailable to you. Want to Upgrade?

To: Dr. Bob who wrote (790)	7/12/1998 10:09:00 PM
From: Prospector	Read Replies (1) \| Respond to of 6039

Thank you Dr White We are trying to keep as much information as we can for everyone to review.. Positive or negative.. helps us keep up with the constant flow of information. Prospector

To: Dr. Bob who wrote (790)	7/13/1998 12:20:00 AM
From: chirodoc	Respond to of 6039

thermography, apparently without alogorithms or computers, was still able to measure 1/10 degreeC--add a computer and some software and away we go. J Dermatol Sci 1998 Mar;16(3):173-181 Effects of nicotine on peripheral cutaneous blood flow and skin temperature. Usuki K, Kanekura T, Aradono K, Kanzaki T Department of Dermatology, Kagoshima University Faculty of Medicine, Miyazaki, Japan. [Medline record in process] We hypothesized that if nicotine was used in a form that was not adulterated with other hazardous substances found in tobacco, it would increase cutaneous blood flow (CBF) resulting in an increase in skin temperature. The effects of nicotine on CBF was investigated in 80 healthy volunteers and 6 patients with peripheral circulation disturbances. Each subject was required to chew nicotine gum (containing 2 mg nicotine) for 15 min and the CBF was then measured with laser blood flowmetry. Skin temperature of 35 volunteers was measured with thermography before and after chewing the gum for 15 min. A control study was performed using ordinary gum without nicotine. Increased CBF (> or = +1 ml/min/100 g) was observed in 55 of 86 subjects (64%, 33.7-38.6 ml/min/100 g, P < 0.01). An elevation in skin temperature (> +0.1 degree C) was also observed with nicotine gum in 26 of 35 healthy subjects (74%, +0.62 +/- 0.96 degree C, P < 0.001). The increase in CBF was greater in subjects in which the initial CBF was lower than in others (P < 0.01). Nicotine gum was found to increase CBF (55/86) and elevate skin temperature (26/35). The smaller the initial CBF value, the greater was the increase in CBF. Nicotine or nicotine derivatives might prove to be useful agents for the treatment of peripheral circulation disturbances.

To: Dr. Bob who wrote (790)	7/13/1998 12:25:00 AM
From: chirodoc	Respond to of 6039

this one everyone should read..... the thermography could measure therogenesis--the heat generated by cell activity--the same thing it measures for cancer detection. .....at .002 C degrees!!!!! Pharm Res 1998 Jun;15(6):944-949 Development of infrared imaging to measure thermogenesis in cell culture: thermogenic effects of uncoupling protein-2, troglitazone, and beta-adrenoceptor agonists. Paulik MA, Buckholz RG, Lancaster ME, Dallas WS, Hull-Ryde EA, Weiel JE, Lenhard JM Department of Metabolic Diseases, GlaxoWellcome Inc., Research Triangle Park, North Carolina 27709, USA. map16209@glaxowellcome.com [Medline record in process] PURPOSE: Although the effects of thermogenic agents in cell culture can be measured by direct microcalorimetry, only a few samples can be analyzed over several hours. In this report, we describe a robust non-invasive technique to measure real-time thermogenesis of cells cultured in microtiter plates using infrared thermography. METHODS: Yeast were transformed with uncoupling protein-2 (UCP2) or exposed to carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or rotenone. Adipocytes were exposed to rotenone, FCCP, cycloheximide. troglitazone, or CL316243. Thermogenesis was measured using infrared thermography. RESULTS: Thermogenesis increased after exposing yeast to the mitochondrial uncoupler, FCCP, or transforming the cells with UCP2. Further, thermogenesis in adipocytes was stimulated by CL316243, a beta3-adrenoceptor agonist being developed to treat obesity. The protein synthesis inhibitor, cycloheximide, did not inhibit CL316243-mediated thermogenesis. In contrast, the mitochondrial proton transport inhibitor, rotenone, inhibited thermogenesis in yeast and adipocytes. Similarly, the antidiabetic agent, troglitazone, suppressed thermogenesis in adipocytes. Although increased UCP synthesis resulted in increased thermogenesis in yeast, UCP expression did not correlate with thermogenesis in adipocytes. CONCLUSIONS: The results, taken together with the high resolution (0.002 degrees C) and robustness (384-well format) of the approach, indicate infrared-imaging is a rapid and effective method for measuring thermogenesis in vitro.