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To: Graham Marshman who wrote (1714)	7/21/1998 9:11:00 PM
From: Cavalry	Respond to of 4028

do your own dd "This new-found evidence that our telomere elongation process could significantly effect cancer therapy is indeed profound. Our cell culture experiments are scheduled for the first quarter of 1998 with animal studies immediately thereafter." said CSI Chairman Dell Gibson from press release last year Cav

To: Graham Marshman who wrote (1714)	7/21/1998 9:16:00 PM
From: Cavalry	Read Replies (1) \| Respond to of 4028

more dd Other Formats: Links: Order this document Proc Natl Acad Sci U S A 1996 Nov 26;93(24):13902-13907 Cell cycle-regulated generation of single-stranded G-rich DNA in the absence of telomerase. Dionne I, Wellinger RJ Faculte de Medicine, Department de Microbiologie et Infectiologie, Universite de Sherbrooke, QC, Canada. Current models of telomere replication predict that due to the properties of the polymerases implicated in semiconservative replication of linear DNA, the two daughter molecules have one end that is blunt and one end with a short 3' overhang. Telomerase is thought to extend the short 3' overhang to produce long single-stranded overhangs. Recently, such overhangs, or TG1-3 tails, were shown to occur on both telomeres of replicated linear plasmids in yeast. Moreover, indirect evidence suggested that the TG1-3 tails also occurred in a yeast strain lacking telomerase. We report herein a novel in-gel hybridization technique to probe telomeres for single-stranded DNA. Using this method, it is shown directly that in yeast strains lacking the TLC1 gene encoding the yeast telomerase RNA, TG1-3 single-stranded DNA was generated on chromosomal and plasmid telomeres. The single-stranded DNA only appeared in S phase and was sensitive to digestion with a single-strand-specific exonuclease. These data demonstrate that during replication of telomeres, TG1-3 tails can be generated in a way that is independent of telomerase-mediated strand elongation. In wild-type strains, these TG1-3 tails could subsequently serve as substrates for telomerase and telomere binding proteins on all telomeres. MeSH Terms: Base Composition Base Sequence Cell Cycle* DNA Replication* DNA, Fungal/chemistry DNA, Fungal/biosynthesis* DNA, Single-Stranded/chemistry DNA, Single-Stranded/biosynthesis Gene Deletion Genes, Fungal Guanine* Oligodeoxyribonucleotides Saccharomyces cerevisiae/genetics* Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae/cytology* Support, Non-U.S. Gov't Telomerase/metabolism* Telomerase/genetics* Telomere/physiology Substances: Guanine Oligodeoxyribonucleotides DNA, Single-Stranded DNA, Fungal Telomerase PMID: 8943033, UI: 97098493 -------------------------------------------------------------------------------- the above report in Macintosh PC UNIX Text HTML format documents on this page through Loansome Doc --------------------------------------------------------------------------------

To: Graham Marshman who wrote (1714)	7/21/1998 9:21:00 PM
From: Cavalry	Respond to of 4028

more dd Other Formats: Links: Order this document Mol Cell Biol 1995 Nov;15(11):6128-6138 Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Garvik B, Carson M, Hartwell L Department of Genetics, University of Washington, Seattle 98195, USA. A cdc13 temperature-sensitive mutant of Saccharomyces cerevisiae arrests in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint. The DNA lesions present after a failure of Cdc13p function appear to be located almost exclusively in telomere-proximal regions, on the basis of the profile of induced mitotic recombination. cdc13 rad9 cells dividing at the restrictive temperature contain single-stranded DNA corresponding to telomeric and telomere-proximal DNA sequences and eventually lose telomere-associated sequences. These results suggest that the CDC13 product functions in telomere metabolism, either in the replication of telomeric DNA or in protecting telomeres from the double-strand break repair system. Moreover, since cdc13 rad9 cells divide at a wild-type rate for several divisions at the restrictive temperature while cdc13 RAD9 cells arrest in G2, these results also suggest that single-stranded DNA may be a specific signal for the RAD9 checkpoint. Published erratum appears in Mol Cell Biol 1996 Jan;16(1):457 MeSH Terms: Base Sequence Cloning, Molecular Cyclins/physiology* DNA Replication* DNA-Directed DNA Polymerase/genetics DNA, Fungal/biosynthesis DNA, Single-Stranded/metabolism Epistasis, Genetic Fungal Proteins/physiology* Gene Deletion Genes, Structural, Fungal Molecular Sequence Data Recombination, Genetic Saccharomyces cerevisiae/genetics* Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Telomere/ultrastructure* Substances: rad9 protein Fungal Proteins DNA, Single-Stranded DNA, Fungal Cyclins Cyclin B DNA-Directed DNA Polymerase Secondary source id: GENBANK/M76550 Grant support: GM17709/GM/NIGMS PMID: 7565765, UI: 96025993 -------------------------------------------------------------------------------- the above report in Macintosh PC UNIX Text HTML format documents on this page through Loansome Doc --------------------------------------------------------------------------------

To: Graham Marshman who wrote (1714)	7/21/1998 9:24:00 PM
From: Cavalry	Read Replies (2) \| Respond to of 4028

more dd Other Formats: Links: Order this document Proc Natl Acad Sci U S A 1994 May 24;91(11):4658-4662 Single-strand conformation polymorphism analysis coupled with stratified DNA sequencing reveals reduced sequence variation in the su(s) and su(wa) regions of the Drosophila melanogaster X chromosome. Aguade M, Meyers W, Long AD, Langley CH Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Spain. Single-strand conformation polymorphism (SSCP) analysis followed by DNA sequencing of stratified sub-samples was used to survey DNA polymorphism in the su(s) and su(wa) regions in a natural population of Drosophila melanogaster. su(s) and su(wa) are located near the telomere of the X chromosome, where the rate of crossing over per kilobase of DNA monotonically decreases toward the tip. SSCP was assessed in 12 noncoding segments amplified from the su(s) region (3213 bp) and in 8 noncoding segments amplified from the su(wa) region (1955 bp). Sets of segments were multiplexed in a single electrophoretic lane to increase the number of base pairs assayed per lane. Eight segments were monomorphic, and the other 12 segments exhibited two to four SSCP classes. Only four within-SSCP-class DNA sequence differences (a single nucleotide substitution) were observed among 24,360 bp compared within classes. The between-SSCP-class DNA sequence comparisons revealed 27 substitutions and 9 insertion/deletion polymorphisms. The average numbers of substitutional differences per site were 0.0010 and 0.0021 for su(s) and su(wa), respectively. These values are intermediate between those reported for the more distal y-ASC region (0.0004) and the more proximal Pgd locus (0.0024). This observation is consistent with the prediction of the hitchhiking-effect model-i.e., a monotonic increase in polymorphism as a function of crossing over per kilobase. MeSH Terms: Animal Base Sequence Crossing Over (Genetics) Drosophila melanogaster/genetics* DNA, Single-Stranded/chemistry* Evolution Molecular Sequence Data Nucleic Acid Conformation Polymorphism (Genetics)* Sequence Analysis, DNA Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Telomere Variation (Genetics) X Chromosome* Substances: DNA, Single-Stranded Secondary source id: GENBANK/X06589 GENBANK/M57889 PMID: 8197115, UI: 94255386 -------------------------------------------------------------------------------- the above report in Macintosh PC UNIX Text HTML format documents on this page through Loansome Doc --------------------------------------------------------------------------------