[High-throughput microarray detection of olfactory receptor gene expression in the mouse ]
>>Published online before print September 17, 2004
Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0405350101
High-throughput microarray detection of olfactory receptor gene expression in the mouse
Xinmin Zhang *, Matthew Rogers *, Huikai Tian , Xiaohong Zhang *, Dong-Jing Zou *, Jian Liu , Minghong Ma , Gordon M. Shepherd , and Stuart J. Firestein *¶
*Department of Biological Sciences, Columbia University, New York, NY 10027; Section of Neurobiology, Yale University School of Medicine, New Haven, CT 06510; and Department of Neuroscience, University of Pennsylvania, Philadelphia, PA 19104
Communicated by Linda B. Buck, Fred Hutchinson Cancer Research Center, Seattle, WA, August 6, 2004 (received for review February 7, 2004)
The large number of olfactory receptor genes necessitates high throughput methods to analyze their expression patterns. We have therefore designed a high-density oligonucleotide array containing all known mouse olfactory receptor (OR) and V1R vomeronasal receptor genes. This custom array detected a large number of receptor genes, demonstrating specific expression in the olfactory sensory epithelium for 800 OR genes previously designated as ORs based solely on genomic sequences. The array also enabled us to monitor the spatial and temporal distribution of gene expression for the entire OR family. Interestingly, OR genes showing spatially segregated expression patterns were also segregated on the chromosomes. This correlation between genomic location and spatial expression provides unique insights about the regulation of this large family of genes.<<
Of interest to followers of Senomyx (SNMX). Don't all raise your hands at once.
Cheers, Tuck |
