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Biotech / Medical : Coronavirus - Covid 19 Information Sharing Forum

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Saulamanca
To: golfer72 who wrote (5057)1/10/2024 8:33:29 AM
From: pheilman_3 Recommendations   of 5937
 
Kory, the inventor of PCR, pointed out it was not useful for diagnosis. They are running a Quantitative Polymerase Chain Reaction, QPCR, after each duplication they check for fluorescence. They would always know on which cycle they got a positive result. This should be reported, rather than just the presence of the DNA. Quantitative. A number. They refused to report that number.

The PCR process is not perfectly accurate, on each thermal cycle some DNA binds to a slight mismatch. That gets duplicated. At 40 cycles, there will be fluorescence, no matter what was in the original mix. 2 to the 40th power is about 1 trillion. Like turning the volume up very high between radio stations, you will hear static.
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