Silicon Investor (SI) -- The First Internet Community

STOCKTALK

We've detected that you're using an ad content blocking browser plug-in or feature. Ads provide a critical source of revenue to the continued operation of Silicon Investor. We ask that you disable ad blocking while on Silicon Investor in the best interests of our community. If you are not using an ad blocker but are still receiving this message, make sure your browser's tracking protection is set to the 'standard' level.

Biotech / Medical : Pharmacyclics (PCYC) -- Ignore unavailable to you. Want to Upgrade?

To: keokalani'nui who wrote (684)	2/20/2002 11:49:39 PM
From: scaram(o)uche	Respond to of 717

eeeeeeeeeeeeeeeeeeeeeewe.............. not nice. :-) You get lotsa brownie points.

To: keokalani'nui who wrote (684)	2/21/2002 12:04:30 PM
From: tnsaf	Read Replies (1) \| Respond to of 717

Here is the abstract from the PNAS article. The text of the article indicates that the infected cells have a level of intracellular oxidative stress caused by the infection and that Gd-Tex increases this, causing infected cells to die. Jason Proc. Natl. Acad. Sci. USA, Vol. 99, Issue 4, 2270-2274, February 19, 2002 Motexafin gadolinium (Gd-Tex) selectively induces apoptosis in HIV-1 infected CD4+ T helper cells (glutathione\|redox modulation) Omar D. Perez,,, Garry P. Nolan,,, Darren Magda§, Richard A. Miller§, Leonard A. Herzenberg¶, and Leonore A. Herzenberg¶, * Department of Microbiology and Immunology, The Baxter Laboratories of Genetic Pharmacology, and Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305-5175; § Pharmacyclics, Sunnyvale, CA 94085; and ¶ Department of Genetics, Stanford University, Stanford, CA 94305-5318 Contributed by Leonard A. Herzenberg, December 29, 2001 Here, we show that motexafin gadolinium (Gd-Tex), a compound that promotes intracellular oxidative stress, selectively induces apoptosis in HIV-1-infected CD4+ T cells in IL-2-stimulated cultures of peripheral blood mononuclear cells infected in vitro with HIV-1. This selective induction of apoptosis, which we detect by FACS analysis of intracellular HIV/p24 and concomitant surface and apoptosis marker expression, is abrogated by the glutathione precursor, N-acetyl-L-cysteine. Importantly, it occurs at Gd-Tex concentrations that are not cytotoxic to uninfected cells in the culture. These findings suggest that Gd-Tex may have therapeutic utility as an anti-HIV agent capable of selectively targeting and removing HIV-infected cells in an infected host. ------------------------------------------------------------ To whom reprint requests should be addressed. E-mail: lenherz@darwin.stanford.edu.