Here's a relevant patent issued in January:
United States Patent
5,712,094
Seidel, et. al.
Jan. 27, 1998
Methods for detecting modulators of cytokine action
Inventors:
Seidel; H. Martin (San Diego, CA); Lamb; I. Peter (San Diego, CA); Chan; Shin-Shay Tian (San Diego,
CA).
Assignee:
Ligand Pharmaceuticals, Inc. (San Diego, CA).
Appl. No.:
411,020
Filed:
Mar. 27, 1995
Intl. Cl. :
C12Q 1/68
Current U.S. Cl.:
435/6; 435/252.3; 435/320.1; 435/325; 536/23.1; 935/33;
935/34; 935/36; 935/70
Field of Search:
435/6, 240.2, 252.3, 320.1, 325; 536/23.1; 935/33, 34, 36,
70
References Cited | [Referenced By]
Foreign Patent Documents
487298A2
Nov., 1991
EP
692488A2
Jan., 1996
EP
WO9213091
Aug., 1992
WO
9508001
Mar., 1995
WO
9528482
Oct., 1995
WO
9528492
Oct., 1995
WO
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Primary Examiner: Ulm; John
Assistant Examiner: Mertz; Prema
Attorney, Agent or Firm: Elmer; J. Scott
Abstract
The present invention provides DNA constructs that contain oligonucleotide sequences comprising DNA regulatory elements
of the general sequence TTN(x) AA that bind activated transcriptional regulatory proteins in response to signaling molecules,
such as cytokines, an operably linked promoter and operably linked heterologous gene. The present invention also provides
host cells transfected with such DNA constructs, as well as methods for measuring the ability of compounds to act as agonists
and antagonists of gene transcription utilizing these DNA constructs and transfected host cells.
4 Claims, 2 Drawing Figures |
